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Research Paper

Journal of the Optical Society of Korea 2009; 13(3): 335-340

Published online September 25, 2009 https://doi.org/10.3807/JOSK.2009.13.3.335

Copyright © Optical Society of Korea.

Ultrashort Pulsed Laser Machining for Biomolecule Trapping

Hae-Woon Choi1, Dave F. Farson2, L.James Lee3, and Ho Lee4

1School of Mechanical and Automotive Engineering, Keimyung University; 2Laboratory for Multiscale Processing & Characterization, The Ohio State University; 3Department of Chemical and Biomolecular Engineering, The Ohio State University; 4School of Mechanical Engineering, Kyungpook National University

Received: June 26, 2009; Revised: September 10, 2009; Accepted: September 14, 2009

Ultrashort pulse laser drilling of polycarbonate track-etched membrane (pTEM) material was used to fabricate a mouse embryo cell trapping device. Holes with a diameter of $2{\mu}m$ to $5{\mu}m$ were fabricated on a $10{\mu}m$ thick membrane using a femtosecond laser with a 150 fs pulse width and 775 nm wavelength and multiple-pulse irradiation. In cell trapping tests, the overall cell occupancy of the machined holes in the fabricated pTEM was found to be more than 80%. The results of a single pulse and multiple pulse irradiation were compared in terms of the surface quality. It was generally found that a single pulse with high energy was less desirable than irradiation with multiple pulses of lower energy.

Keywords: Ultrashort laser, Femtosecond laser, Cell trapping, pTEM,

OCIS codes: 140.0140; 170.7160

  1. B. Stuart, M. Feit, A. Rubenchik, B. Shore, and M. Perry, "Laser-induced damage in dielectrics with nanosecond to subpicosecond pulses," Physical Review Letters 74, 2248-2252 (1995)
    CrossRef
  2. S. Nolte, C. Momma, H. Jacobs, A. Tunnermann, B. Chichkov, B. Wellegehausen, and H. Welling, "Ablation of metals by ultrashort laser pulses," J. Opt. Soc. Am. B 14, 2716-2722 (1997)
    CrossRef
  3. J. Acker, "Bio-preservation of cells and engineered tissues," in Tissue Engineering II, T. Scheper, K. Lee, and D. Kaplan, ed. (Springer, Berlin, Germany, 2005)
    CrossRef
  4. A. Garner, G. Chen, N. Chen, V. Sridhara, J. Kolb, R. Swanson, S. Beebe, R. Joshi, and K. Schoenbach, "Ultrashort electric pulse induced changes in cellular dielectric properties," Biochem. Biophy. Research Comm. 362, 139-144 (2007)
    CrossRef
  5. X. Liu, D. Du, and G. Mourou, "Laser ablation and micromachining with ultrashort laser pulses," IEEE J. Quantum Electron. 33, 1706-1716 (1997)
    CrossRef
  6. D. Du, X. Liu, G. Korn, J. Squier, and G. Mourou, "Laser induced breakdown by impact ionization in SiO2 with pulse widths from 7ns to 150fs," Appl. Phys. Lett. 64, 3071-3073 (1994)
    CrossRef
  7. V. Kohli, J. Acker, and A. Elezzabi, "Reversible permeabilization using high-intensity femtosecond laser pulses: applications to biopreservation," Biotechnol. Bioeng. 92, 889-899 (2005)
    CrossRef
  8. L. MacQueen, M. Bushmann, and M. Wertheimer, "Gene delivery by electroporation after dielectro-phoretic positioning of cells in a non-uniform electric field," Bioelectrochemistry 72, 141-148 (2008)
    CrossRef
  9. J. Zhang, P. Blackmore, B. Hargrave, S. Xiao, S. Beebe, and K. Schoenbach, "Nanosecond pulse electric field: a novel non-ligand agonist for platelet activation," Archives of Biochemistry and Biophysics 471, 240-248(2008)
    CrossRef
  10. H. Wang and C. Lu, "Microfluidic electroporation for delivery of small molecules and genes into cells using a common DC power supply," Biotechnol. Bioeng. 100, 579-586 (2008)
    CrossRef
  11. Wikipedia, http://en.wikipedia.org/wiki/Electroporation
  12. http://www.whatman.com/
  13. http://www.2spi.com/catalog/spec˙prep/filter3.html
  14. M. Sasnett, "Propagation of multimode laser beams - the M2 factor," in The Physics and Technology of Laser Resonators, D. Hall and P. Jackson, ed. (IOP Publishing, Bristol, UK, 1989), pp. 132-142
  15. K. Salonitis, A. Stournaras, G. Tsoukantas, P. Stavropoulos, and G. Chryssolouris, "A theoretical and experimental investigation on limitations of pulsed laser drilling," J. Mat. Proc. Technology 183, 96-103 (2007)
    CrossRef
  16. H. Choi, J. Johnson, J. Nam, D. Farson, and J. Lannutti, "Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation," J. Laser Applications 19, 225-231 (2007)
    CrossRef